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Time points were stable if the mean concentration was at least 0.80 units/m L.
All time points retained at least 90% of the equilibrium concentration, with the exception of hour 168 (0.79 ± 0.03 units/m L).
The time points were stable if the mean concentration of the samples exceeded 90% of the equilibrium concentration at 6 hours.
Results: The equilibrium concentration was 0.89 units/m L.
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ADA notes with sadness the passing of Gerald Jerry Reaven, MD, whose pioneering work in insulin resistance provides critical components of our current understanding of the pathophysiology of type 2 diabetes.
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Methods: At time “0” three admixtures of regular human insulin were prepared by withdrawing 1 m L of regular human insulin with a concentration of 100 units/m L and adding it to a sufficient quantity of 0.9% sodium chloride for injection in a polyvinylchloride bag to yield a total volume of 100 m L.The three admixtures were stored under refrigeration (2°C–8°C [36°F–46°F]), and one sample of each admixture was withdrawn and tested in duplicate at 0, 6, 24, 48, 72, 144, 168, 192, 216, 240, 312, and 336 hours.Utilizing high performance liquid chromatography, each sample underwent immediate testing.At 192 hours the mean concentration was 0.88 ± 0.03 units/m L.At 336 hours the mean concentration was 0.91 ± 0.02 units/m L.